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The Analysis of Genetic Diversity of Djulis (Chenopodium formosanum Koidz.) Using Double Digest RADseq1

2020-08-03

The Analysis of Genetic Diversity of Djulis (Chenopodium formosanum Koidz.)
Using Double Digest RADseq1

Tzu-Yun Huang2


Abstract

Djulis (Chenopodium formosanum Koidz.) is an important specialty cereal crop in Taitung. Current cultivars are mostly local lines conserved by aboriginal tribes. Due to long term traditional djulis cultivation habit, local lines are mixed by multiple lines. The related genetics and breeding studies are also insufficient. In order to understand the genetic background among djulis germplasms and to improve the breeding efficiency, this study used 39 djulis accessions collected in Taitung, Hualien, and Pingtung by Taitung District Agricultural Research and Extension Station in 2009 as the material, to understand the relationship between djulis accessions. We used double-digest RAD sequencing to build the library, resulting 1,046 polymorphic loci for genetic structure analysis and principal coordinate analysis. The sequencing quantity of 98T011 accession was not enough and was excluded from the following analysis. Genetic structure analysis was analyzed by STRUCTURE software. When K=3, 38 djulis accessions had the maximum ΔK, indicated that K=3 was the best cluster number of subpopulation. Principal coordinate anlysis was analyzed by R software. The analysis result showed that 38 djulis accessions were divided into two groups according to the region they had been collected. The genetic background of the accessions 98T020 was different from other accessions, and was classified to another groups. The result of this study providing the strategy for djulis breeding and germplasm conservation.

 

Key words: Djulis, Accession, ddRAD, Genetic diversity


1The research bulletin is a part of the master thesis of the author.

2Assistant Researcher of Taitung DARES, COA.